Primer

Part:BBa_K4496009:Design

Designed by: Ana Paula Contelli Xavier, Larissa de Souza Kawanisi   Group: iGEM22_USP-EEL-Brazil   (2022-09-20)


Primer (REV) optimized for Bj46a gene amplification in Pichia pastoris


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This primer was designed from the Bj46a sequence optimized for Pichia Pastoris. To do so, the last 8 amino acids were chosen, the primer was designed and tested for hairpin.


Source

https://parts.igem.org/Part:BBa_K4496002

References